Issues

• assembly edge case not supported since pydna v5.5.14

  OpenCloning/OpenCloning_backend#497 · Filter by author manulera opened on Jun 5, 2026

• Primer binding functionality to find off-targets

  OpenCloning/OpenCloning_backend#456 · Filter by author manulera opened on May 15, 2026

• Test wrong credentials addgene

  OpenCloning/OpenCloning_backend#434 · Filter by author manulera opened on Apr 10, 2026

• Clarify assembly topology beyond circular

  OpenCloning/OpenCloning_backend#420 · Filter by author manulera opened on Mar 17, 2026

• Refactor homologous_recombination endpoint to allow single inputs for excision, and multiple insertion fragments

  OpenCloning/OpenCloning_backend#368 · Filter by author manulera opened on Oct 20, 2025
  1

• Review blunt handling in known assembly

  OpenCloning/OpenCloning_backend#353 · Filter by author manulera opened on Sep 2, 2025

• Handle only_adjacent option for restriction/ligation

  OpenCloning/OpenCloning_backend#352 · Filter by author manulera opened on Sep 2, 2025

• Allowlist error Genbank gives internal server error

  OpenCloning/OpenCloning_backend#340 · Filter by author manulera opened on Jul 1, 2025

• httpclient allowlist error > change to requestError?

  OpenCloning/OpenCloning_backend#339 · Filter by author manulera opened on Jul 1, 2025

• Wrong circular alignment

  OpenCloning/OpenCloning_backend#336 · Filter by author manulera opened on Jun 20, 2025

• Should this type of insertion assemble be allowed?

  question
  OpenCloning/OpenCloning_backend#331 · Filter by author manulera opened on Jun 17, 2025
  2

• Adding Cas12-like Nucleases

  OpenCloning/OpenCloning_backend#328 · Filter by author alfredbrown1 opened on Jun 16, 2025

• Review format_known_assembly_response

  OpenCloning/OpenCloning_backend#307 · Filter by author manulera opened on May 26, 2025

• Handle NCBI taking too long to respond

  OpenCloning/OpenCloning_backend#294 · Filter by author manulera opened on Apr 22, 2025

• Ziqiang et al. template - change one template to 50590?

  OpenCloning/OpenCloning_backend#293 · Filter by author manulera opened on Apr 14, 2025

• Script documentation: mention origin-spanning features

  OpenCloning/OpenCloning_backend#288 · Filter by author manulera opened on Mar 25, 2025

• Migrate assembly code to pydna

  OpenCloning/OpenCloning_backend#282 · Filter by author manulera opened on Mar 17, 2025
  1

• Test EBIC primers

  OpenCloning/OpenCloning_backend#277 · Filter by author manulera opened on Feb 27, 2025

• Store overhang info in gb file

  OpenCloning/OpenCloning_backend#276 · Filter by author manulera opened on Feb 24, 2025

• permutate_trace: improve discriminating between Sanger and full-plasmid sequence

  OpenCloning/OpenCloning_backend#270 · Filter by author manulera opened on Feb 19, 2025

• Allow cut-through slicing of features

  OpenCloning/OpenCloning_backend#260 · Filter by author manulera opened on Feb 4, 2025

• Better error handling for external requests

  OpenCloning/OpenCloning_backend#253 · Filter by author manulera opened on Dec 17, 2024

• Primer design for point mutations

  OpenCloning/OpenCloning_backend#233 · Filter by author dgruano opened on Dec 3, 2024

• test pLannotate timeout

  OpenCloning/OpenCloning_backend#226 · Filter by author manulera opened on Nov 26, 2024

• Support for genome editing

  OpenCloning/OpenCloning_backend#187 · Filter by author dgruano opened on Sep 10, 2024
  4